解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::A kinetic study was performed of a model for an autocatalytic zymogen activation process involving both intra- and intermolecular routes, to which a chromogenic reaction in which the active enzyme acts upon one of its substrates was coupled to continuously monitor the reaction. Kinetic equations describing the evoluti...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2005.080
更新日期:2005-07-01 00:00:00
abstract::The enzyme L-asparaginase is a crucial component in the treatment of acute lymphoblastic leukemia (ALL). As all asparaginases in clinical use are derived from microorganisms, immunological reactions are the most important adverse events associated with asparaginase treatment. Two different methods, phage display and t...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2005.063
更新日期:2005-06-01 00:00:00
abstract::The heterodimer consisting of ecdysteroid receptor (EcR) and ultraspiracle (USP), both of which are members of the nuclear receptor superfamily, is considered to be the functional ecdysteroid receptor. Here we analyzed the subcellular distribution of EcR and USP fused to fluorescent proteins. The experiments were carr...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2005.055
更新日期:2005-05-01 00:00:00
abstract::Transforming growth factor-beta1 (TGF-beta1) has a biphasic effect on the growth of renal epithelial cells. In transformed cells, TGF-beta1 appears to accelerate the proliferation of malignant cells. The diverse cellular functions of TGF-beta1 are regulated by three high-affinity serine/threonine kinase receptors, nam...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2005.045
更新日期:2005-04-01 00:00:00
abstract::The cell, as a molecular system, is often interpreted in terms of complex clockworks, and the design charts of mechanical and electrical engineering are assumed to provide adequate approximations for the description of cellular organization. However, a growing body of experimental evidence obtained through the observa...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2005.030
更新日期:2005-03-01 00:00:00
abstract::While the role of C2-ceramide in the induction of programmed cell death (PCD) in animal systems has been well documented, little is known of its role in plant cells. Here we show that C2-ceramide induces PCD in Arabidopsis suspension cultures, which is preceded by the generation of a calcium transient and an increase ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2005.020
更新日期:2005-02-01 00:00:00
abstract::The thyroglobulin type-1 (Tg-1) domain is a protein module that occurs in a variety of secreted and membrane proteins and is recognised as a potent inhibitor of cysteine peptidases. We present here some properties of the Tg-1 domain of human testican, a modularly organised proteoglycan secreted mainly by brain cells, ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2005.010
更新日期:2005-01-01 00:00:00
abstract::Insulin contains three disulfide bonds, one intrachain bond, A6-A11, and two interchain bonds, A7-B7 and A20-B19. Site-directed mutagenesis results (the two cysteine residues of disulfide A7-B7 were replaced by serine) showed that disulfide A7-B7 is crucial to both the structure and activity of insulin. However, chemi...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2004.151
更新日期:2004-12-01 00:00:00
abstract::Pseudomonas aeruginosa and Serratia marcescens can cause refractory keratitis resulting in corneal perforation and blindness. These bacteria produce various kinds of proteases. In addition to pseudomonal elastase (LasB) and alkaline protease, LasA protease and protease IV have recently been found to be more important ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2004.131
更新日期:2004-11-01 00:00:00
abstract::Plasma kallikrein plays a role in coagulation, fibrinolysis and inflammation. Cathepsins B and L participate in (patho)physiological processes such as peptide antigen processing, tissue remodeling events, protein turnover in cells, hormone processing and tumor invasion. The present work analyzes the processing of prek...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2004.141
更新日期:2004-11-01 00:00:00
abstract::The surface topography of red blood cells (RBCs) was investigated under near-physiological conditions using atomic force microscopy (AFM). An immobilization protocol was established where RBCs are coupled via molecular bonds of the membrane glycoproteins to wheat germ agglutinin (WGA), which is covalently and flexibly...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2004.124
更新日期:2004-10-01 00:00:00
abstract::MHC (major histocompatibility complex) class I molecules bind intracellular virus-derived peptides in the endoplasmic reticulum (ER) and present them at the cell surface to cytotoxic T lymphocytes. Peptide-free class I molecules at the cell surface, however, could lead to aberrant T cell killing. Therefore, cells ensu...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2004.100
更新日期:2004-09-01 00:00:00
abstract::Glucose-dependent insulinotropic polypeptide (GIP) is an incretin hormone secreted by endocrine K-cells in response to nutrient absorption. In this study we have utilized a specific and enzymatically stable GIP receptor antagonist, (Pro3)GIP, to evaluate the contribution of endogenous GIP to insulin secretion and gluc...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2004.110
更新日期:2004-09-01 00:00:00
abstract::The conversion of cellular prion protein (PrP(C)) into its pathological isoform (PrP(Sc)) conveys an increase in hydrophobicity and induces a partial resistance to proteinase K (PK). Interestingly, co-incubation with high copper ion concentrations also modifies the solubility of PrP(c) and induces a partial PK resista...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2004.090
更新日期:2004-08-01 00:00:00
abstract::Group II introns are autocatalytic RNAs which self-splice in vitro. However, in vivo additional protein factors might be involved in the splicing process. We used an affinity chromatography method called 'StreptoTag' to identify group II intron binding proteins from Saccharomyces cerevisiae. This method uses a hybrid ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2004.076
更新日期:2004-07-01 00:00:00
abstract::The infectivity and pathogenicity of influenza virus are primarily determined by host cellular trypsin-type processing proteases which cleave the viral membrane fusion glycoprotein hemagglutinin (HA). Therefore the distribution of the processing protease is a major determinant of the infectious organ tropism. The comm...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2004.057
更新日期:2004-06-01 00:00:00
abstract::Dipeptidyl peptidase-IV has been demonstrated to play a role in cancer biology by many authors. Since then, additional proteins possessing similar enzymatic activity have been described and their role in cancerogenesis has been hypothesized. To assess the complexity of these 'Dipeptidyl peptidase-IV activity and/or st...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2004.067
更新日期:2004-06-01 00:00:00
abstract::LFM-A13, or alpha-cyano-beta-hydroxy-beta-methyl-N-(2,5-dibromophenyl)propenamide, was shown to inhibit Bruton's tyrosine kinase (Btk). Here we show that LFM-A13 efficiently inhibits erythropoietin (Epo)-induced phosphorylation of the erythropoietin receptor, Janus kinase 2 (Jak2) and downstream signalling molecules. ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2004.045
更新日期:2004-05-01 00:00:00
abstract::Two current hypotheses of O2 sensing in the carotid body (CB) chemoreceptors suggest participation of oxygen reactive (ROS) species, but they are mechanistically opposed. One postulates that hypoxia decreases ROS levels; the other that hypoxia increases them. Yet, both propose that the ensuing alteration in the cellul...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2004.021
更新日期:2004-03-01 00:00:00
abstract::Recent reports presented contradictory results regarding the catabolism of mature atrial (ANP) and brain (BNP) natriuretic peptides in circulation. Especially the role of neutral endopeptidase (NEP) in BNP degradation was conversely discussed. Our present in vitro-studies characterize the NEP-dependent metabolism of A...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2004.036
更新日期:2004-02-01 00:00:00
abstract::This review addresses many of the chemical aspects of nitrosative stress mediated by N2O3. From a cellular perspective, N2O3 and the resulting reactive nitrogen oxide species target specific motifs such as thiols, lysine active sites, and zinc fingers and is dependant upon both the rates of production as well as consu...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2004.001
更新日期:2004-01-01 00:00:00
abstract::Fluorescence correlation spectroscopy (FCS) was used in monitoring human parvovirus B19 virus-like particle (VLP) antibody complexes from acute phase and past-immunity serum samples. The Oregon Green 488-labeled VLPs gave an average diffusion coefficient of 1.7 x 10(-7) cm2 s(-1) with an apparent hydrodynamic radius o...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2004.011
更新日期:2004-01-01 00:00:00
abstract::Glutaminyl cyclases (QC) catalyze the intramolecular cyclization of N-terminal glutamine residues of peptides and proteins. For a comparison of the substrate specificity of human and papaya QC enzymes, a novel continuous assay was established by adapting an existing discontinuous method. Specificity constants (kcat/Km...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.175
更新日期:2003-12-01 00:00:00
abstract::Low NO concentrations synthesized by constitutively expressed NO synthases act on several signaling pathways activating transcription factors (TF), such as NF-kappaB or AP-1, and thereby influence gene expression. In contrast, during inflammatory reactions the inducible NO synthase produces NO for prolonged periods of...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2003.153
更新日期:2003-10-01 00:00:00
abstract::The occurrence of rhodanese-like proteins in the major evolutionary phyla, together with the observed abundance of these proteins also within the same genome, suggests that their function cannot be limited to cyanide scavenging. The aim of the present study was to investigate whether Azotobacter vinelandii RhdA, an en...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.163
更新日期:2003-10-01 00:00:00
abstract::RIO1 is an essential gene that encodes a protein serine kinase and is transcribed constitutively at a very low level. Transcriptional activation of RIO1 dispenses with a canonical TATA box as well as with classical transactivators or specific DNA-binding factors. Instead, a dG-dC-rich sequence element, that is located...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.143
更新日期:2003-09-01 00:00:00
abstract::Fibroblast growth factor (FGF) receptor substrate 2 (FRS2) is a membrane-anchored docking protein that has been shown to play an important role in linking FGF, nerve growth factor (NGF) and glial cell-derived neurotrophic factor (GDNF) receptors with the Ras/mitogen-activated protein (MAP) kinase signaling cascade. He...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.134
更新日期:2003-08-01 00:00:00
abstract::The O-acetylation of sialic acids turns out to be one of the most important modifications that influence the diverse biological and pathophysiological properties of glycoconjugates in animals and microorganisms. To understand the functions of this esterification, knowledge of the properties, structures and regulation ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.116
更新日期:2003-07-01 00:00:00
abstract::Recent progress in the identification and partial characterization of novel genes encoding cysteine proteases of the papain family has considerably increased our knowledge of this family of enzymes. Kinetic data available to date for this large family indicate relatively broad, overlapping specificities for most enzym...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2003.094
更新日期:2003-06-01 00:00:00
abstract::Components of kinin-forming systems operating at inflammatory sites are likely to interact with elastase that is released by recruited neutrophils and may, at least temporarily, constitute the major proteolytic activity present at these sites. The aim of this work was to determine the effect of kininogen degradation b...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.104
更新日期:2003-06-01 00:00:00
abstract::In order to enhance and target gene delivery we have previously established a novel method, termed magnetofection, which uses magnetic force acting on gene vectors that are associated with magnetic particles. Here we review the benefits, the mechanism and the potential of the method with regard to overcoming physical ...
journal_title:Biological chemistry
pub_type: 杂志文章,评审
doi:10.1515/BC.2003.082
更新日期:2003-05-01 00:00:00
abstract::Meprins are zinc-endopeptidases of the astacin family, which are expressed as membrane-bound or secreted forms in renal and intestinal brush-border membranes of mouse, rat and man. There are two types of meprin subunits, alpha and beta, which form disulfide-bonded homo- and heterodimers; further oligomerization is med...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.092
更新日期:2003-05-01 00:00:00
abstract::Interleukin-1 signaling is initiated by recruitment of adapter proteins and kinases to the type I interleukin-1 receptor (IL-1RI). It is modulated by accompanying redox processes at various levels, such as (auto-) phosphorylation of the IL-1RI-associated kinase IRAK, the phosphorylation of IkappaB and translocation an...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.068
更新日期:2003-04-01 00:00:00
abstract::The ubiquitous calpains, mu- and m-calpain, have been implicated in essential physiological processes and various pathologies. Cell-permeable specific inhibitors are important tools to elucidate the roles of calpains in cultivated cells and animal models. The synthetic N-acetylated 27-mer peptide derived from exon B o...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.045
更新日期:2003-03-01 00:00:00
abstract::Bauhinia ungulata factor Xa inhibitor (BuXI) inactivates factor Xa and LOPAP, a prothrombin activator proteinase isolated from the venom of Lonomia obliqua caterpillar bristles. The reactive site of the enzyme-inhibitor interaction was explored to design specific substrates for both enzymes. Methionine is crucial for ...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.055
更新日期:2003-03-01 00:00:00
abstract::Full-length Hsp70 protein (Hsp70) and the C-terminal domain of Hsp70 (Hsp70C) both stimulate the cytolytic activity of naive natural killer (NK) cells against Hsp70-positive tumor target cells. Here, we describe the characterization of Hsp70-NK cell interaction with binding studies using the human NK cell line YT. Bin...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.030
更新日期:2003-02-01 00:00:00
abstract::Two members of the nuclear receptor superfamily, EcR (ecdysteroid receptor protein) and Usp (Ultraspiracle), heterodimerize to form a functional receptor for the steroid hormone 20-hydroxyecdysone and thus enable it to coordinate morphogenetic events during insect metamorphosis. N-terminally His-tagged Usp was overexp...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.007
更新日期:2003-01-01 00:00:00
abstract::The transcription factor E2F1 plays a decisive role in the G1/S and G2/M checkpoint transitions of proliferating cells. Because cells are arrested at these checkpoints after heat shock it was of interest to test heat shock effects on E2F1 activity. In human A549 cells, heat shock (44 degrees C, 30 min) caused an immed...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2003.017
更新日期:2003-01-01 00:00:00
abstract::HB13 human myoblasts express physiological and biochemical markers associated with myoblast differentiation in non-human cell culture model systems. During differentiation, HB13 myoblasts also demonstrate fusion-related increases in cathepsin B activity and protein levels. These increases are associated with an increa...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2002.207
更新日期:2002-12-01 00:00:00
abstract::Based on its monophenolic structure and given its pharmacological and toxicological importance, the ability of tyrosinase to oxidize acetaminophen was studied for the first time. Progress curves showed a transient phase characteristic of the monophenolase activity of tyrosinase prior to attaining the steady-state. The...
journal_title:Biological chemistry
pub_type: 杂志文章
doi:10.1515/BC.2002.217
更新日期:2002-12-01 00:00:00